Universitäre Service-Einrichtung für Transmissionselektronenmikroskopie
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Optical microscopy

Application of p53-autoantibodies as tumour markers in mammacarcinom tissue
Red regions: bounded antibodies; surroundings: tumour tissue with cell nuclei,
scanned region: 500 mm x 500 mm.

Besides classical optical microscopy USTEM offers its customers also access to confocal microscopy.

Confocal microscopy (laser scanning microscopy)

is an imaging technique that eliminates out-of-focus light in specimens and enables 3-D imaging of thick specimens. A single focused point of laser illumination (spot or slit) is scanned across a specimen to create optical sections down to 250 nm thickness using visible light. Signals pass back to a photomultiplier detector through a pinhole aperture, which blocks light from other regions of the specimen. These optical sections may be stacked to provide a 3-D digital reconstruction of the specimen.

The technique can be used with both fixed and live specimens. Dynamic events in cells can be imaged with the help of time-lapse imaging and can be viewed in 3-D.

For more information visit the external web pages:

http://www.nikoninstruments.eu/infocenter.php?n=Confocal

http://www.microscopyu.com/articles/confocal/